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KMID : 0881720220370060373
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Journal of Food Hygiene and Safety
2022 Volume.37 No. 6 p.373 ~ p.384
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Development and Validation of an Analytical Method for Fungicide Fluoxastrobin Determination in Agricultural Products
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Lee So-Eun
Lee Su-Jung
Gu Sun-Young
Park Chae-Young
Shin Hye-Sun
Kang Sung-Eun
Lee Jung-Mi
Chung Yun-Mi
Jang Gui-Hyun
Moon Gui-Im
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Abstract
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Fluoxastrobin a fungicide developed from Strobilurus species mushroom extracts, can be used as an effective pesticide to control fungal diseases. In this study, we optimized the extraction and purification of fluoxastrobin according to its physical and chemical properties using the QuEChERS method and developed an LC-MS/MS-based analysis method. For extraction, we used acetonitrile as the extraction solvent, along with MgSO4 and PSA. The limit of quantitation of fluoxastrobin was 0.01 mg/kg. We used 0.01, 0.1, and 0.5 mg/kg of five representative agricultural products and treated them with fluoxastrobin. The coefficients of determination (R2) of fluoxastrobin and fluoxastrobin Z isomer were > 0.998. The average recovery rates of fluoxastrobin (n=5) and fluoxastrobin Z isomer were 75.5?100.3% and 75.0?103.9%, respectively. The relative standard deviations (RSDs) were < 5.5% and < 4.3% for fluoxastrobin and fluoxastrobin Z isomer, respectively. We also performed an interlaboratory validation at Gwangju Regional Food and Drug Administration and compared the recovery rates and RSDs obtained for fluoxastrobin and fluoxastrobin Z isomer at the external lab with our results to validate our analysis method. In the external lab, the average recovery rates and RSDs of fluoxastrobin and fluoxastrobin Z isomer at each concentration were 79.5?100.5% and 78.8?104.7% and < 18.1% and < 10.2%, respectively. In all treatment groups, the concentrations were less than those described by the ‘Codex Alimentarius Commission’ and the ‘Standard procedure for preparing test methods for food, etc.’. Therefore, fluoxastrobin is safe for use as a pesticide.
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KEYWORD
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Fluoxastrobin, Fluoxastrobin Z isomer, LC-MS/MS, Fungicide, Agricultural Products
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